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-111- Immunology(5) 9/5 Room-A
PROTEOLYTIC ACTIVITY OF A GROUP 3 ALLERGEN OF DERMATOPHAGOIDES FARINAE.
Keishi Maruo, Tomomichi Ono.
Department of Dermatology, Kumamoto University School of Medicine, Kumamoto, Japan.
The group 3 allergen of Dermatophagoides specise (Der f III and Der p III) has been identified as a 30-kDa trypsin like protease of the house dust mite. We examined the potential of Der f III to activate the complement system and bradykinin-generating cascade in a purified system. Der f III showed strong activation of the bradykinin-generating cascade and complement system. Furthermore, we demonstrated that a protease similar to Der f III was found to exist in the house dust extract. We speculate that the proteolytic activity of Der f III may contribute the pathogenesis of allergic and inflammatory diseases caused by house dust allergens.
-112- Immunology(5) 9/5 Room-A
A STUDY OF RAST TITER TO TRICOPHYTON IN PATIENTS WITH ATOPIC DERMATITIS.
Hiroyuki Osuna1, Miho Yamamoto1, Sanami Takahashi1, Rumi Nishida1, Megumi Miyazawa1, Sumi Onuma1, Junko Osawa1, Kazuko Kitamura1, Kosuke Takatori2, Zenro Ikezawa1.
1Department of Dermatology, Yokohama City University, School of Medicine, Urafune Hospital, Yokohama, 2Division of Microbiology, National Institute of Health Science, Tokyo, Japan.
It is suggested that the sensitization to fungi is relevant to chronic, recalcitrant allergic diseases. We analyzed RAST titer to Tricophyton in 59 patients with atopic dermatitis (severity: mild,5 cases; moderate,31 cases; severe,23 cases) and studied cross allergenecity. 83% had positive RAST to Tricophyton. The geometric mean of the RAST is 2.38(UA/ml) in mild cases, 7.72 in moderate cases and 15.91 in severe cases, respectively. The correlation coefficient in the RAST responses is 0.731 between Penicillium and Tricophyton and 0.587 between Candida and Tricophyton. RAST inhibition study indicated cross allergenecity between Candida and Tricophyton.
-113- Immunology(5) 9/5 Room-A
FUNCTIONAL ANALYSIS OF HELPER T CELL SUBSETS IN ATOPIC DERMATITIS PATIENTS.
Yukuhiko Kato, Yukari Okubo, Michiyuki Koga.
Department of Dermatology, Tokyo Medical College, Tokyo, Japan.
Peripheral blood mononuclear cells (PBMC) from atopic dermatitis (AD) are reported to produce decreased levels of Th1 cytokine. We studied the production of IFN-( and IL-4 in PBMCs at the single cell level from AD classified into two groups by serum IgE level. PBMC from 5 high IgE AD (HAD) patients (total IgE>10000U/ml), 5 low IgE AD (LAD) patients(total IgE<150U/ml) and 4 healthy controls were stimulated with phorbol ester, ionomycin and Dermatophagoides farinae (Df). Cells were made permeable, stained for intracellular cytokine IFN-( and IL-4 and surface marker CD4 and fixed. IFN-( producing cells were decreased among CD4+ T cells in HAD stimulated with phorbol ester and ionomycin. The number of IL-4- producing cells tended to increase in HAD stimulated with Df.. No difference was observed between LAD and controls. Our findings suggested an aberration of T cells of HAD in cytokine production, which is not necessarily related to the pathogenesis of AD.
-114- Immunology(5) 9/5 Room-A
ANALYSIS OF THE EXPRESSION OF HEAT SHOCK PROTEIN 72 AND 27 IN THE LESIONAL SKIN OF ATOPIC DERMATITIS.
Mehran Ghoreishi, Hiroo Yokozeki, Kiyoshi Nishioka.
Departement of Dermatology, Tokyo Medical and Dental University, Tokyo, Japan.
Biopsy specimens from skin lesions of atopic dermatitis (AD) ( n=27), contact dermatitis (CD) (n=22), normal skin (NS) (n=10) were studied for expression of HSP72 and HSP27.The expression of HSP72 in epidermal keratinocytes or lymphocytes, in the skin lesions of AD was more intense than skin lesions of CD or normal skin . The expression of HSP27 was nearly same between AD, CD, and normal skin. No infiltrated lymphocyte expressed HSP27 in AD, CD, and normal skin, and no positive lymphocyte expressing HSP72 was seen in CD or normal skin. The positive infiltrated lymphocytes predominantly consist of CD4 positive lymphocyte. These studies suggest that abberant expression of HSP72 in skin lesion of AD might play a role in the pathogenesis of disease.
-115- Immunology(5) 9/5 Room-A
GENETIC ANALYSIS OF ATOPIC DERMATITIS.
Makoto Ichimiya, Masahiko Muto, Akiko Ohmura, Hiroaki Tateno, Yuki Yasui, Chidori Asagami.
Department of Dermatology, Yamaguchi University School of Medicine, Ube, Japan.
In order to elucidate susceptibility to atopic dermatitis, we investigated DNA polymorphisms of HLA-DR allele and of mast cell chymase. This study enrolled 51 patients with AD. Diagnosis of AD was based on the criteria proposed by the Japanese Dermatological Association. The frequency of HLA-DR1 and DR13 allele was significantly increased in AD compared with those of normal controls (31.4% VS 10.8% for DR1; p<0.05, 20.7% VS 10.8% for DR13; p<0.05). We conclude that the susceptibility gene involved in the development of AD might be in linkaga disequilibria with HLA-DR1 and/or DR13. We are examining genetypic polymorphisms of mast cell chymase which might act in the effector phase of the disaese.
-116- Immunology(5) 9/5 Room-A
MAST CELLS IN THE EPIDERMIS OF PATIENTS WITH ATOPIC DERMATITIS.
Shuhei Imayama, Akira Eguchi, Keiichiro Nakamura, Yosaburo Shibata.
Departments of Dermatology and Anatomy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
We observed mast cells in the epidermis (as well as the dermis) of the skin specimens of atopic dermatitis fixed with Carnoy's, but in none of the conventionally fixed specimens. While mast cells have been identified in the connective tissue of the skin, they have not been observed in the epidermis even in patients with atopic dermatitis. Criteria for identifying mast cells included 1) metachromatic response to staining with toluidine blue, 2) positive immunoreaction for anti-human mast cell tryptase monoclonal antibody, and 3) typical ultrastructural features observed on electron microscopy. Observations by immunohistochemistry and electron microscopy confirmed the findings. In all of the specimens, mast cells were seen in the dermal connective tissue, particularly around the capillaries. The fact that special fixation and staining are required to observe mast cells in the epidermis implies that the cells belong to the mucous type. These cells are a source of the cytokines that recruit and activate the inflammatory cells involved in the allergic reaction. Their presence may explain the initiation of the late-phase inflammatory response in the skin of patients with atopic dermatitis.