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-194- Hair Biology(2) 9/5 Room-D
EFFECTS OF IMMUNOSUPPRESSANTS AND STEROID HORMONES ON NEW HAIR GROWTH IN MICE.
Tokuro Iwabuchi, Yoshiharu Tsuji, Tsunao Magara, Masahiro Tajima.
Shiseido Research Center, Yokohama, Japan.
Immunosuppressants, cyclosporin A (CsA) and FK506, induce hair growth in mice [1]. Several immunosuppressant binding proteins associate to hsp90 of the steroid hormone receptor complexes in cytosole. Recently, it has been reported that estrogen inhibits new hair growth in mice, however, estrogen antagonist promotes it [2]. Thus, it is thought to be that immunosuppressants and steroid hormone complexes closely related in hair growth initiation . In this study, we examined the effects of CsA and steroid hormones on hair growth in mice. Male C3H/He mice were used at telogen phase. The ethanol solution of the compounds were sequentially applied for 5 days onto the hair-cut dorsal skin of mice. CsA promotes new hair growth in mice. However, HC, ED and DHT did not promote it. HC (0.1 mM) inhibited hair growth induced by CsA. DHT (100 mM) revealed inhibitory effect on it . ED (100 mM) did not inhibited hair growth by CsA. We will discuss the effects of steroid hormone-antagonists and the role of steroid hormone receptors on hair growth. [1] T. Iwabuchi et al. (1995) J. Dermatol. Sci. 9:64-69 [2] H-S. Oh and R.C. Smart (1996) PNAS 93:12525-12530
-195- Hair Biology(2) 9/5 Room-D
EXPRESSION OF 17b-HYDROXYSTEROID DEHYDROGENASE mRNA IN OUTER ROOT SHEATH CELLS AND DERMAL PAPILLA CELLS.
Yumi Andou, Satoshi Itami, Kunihiko Yoshikawa.
Department of Dermatology, Osaka University Medical School,Osaka, Japan.
It is well known that androgen stimulate the growth of some sorts of human hairs and that dermal papilla cells (DPC) may play a role in androgen-dependent hair growth. In order to know the possible involvement of adrenal androgens in human hair growth, we studied the expression of 17b-hydroxysteroid dehydrogenase mRNA in outer root sheath cells (ORS) and DPC from various tissue. The mRNA of type II 17b-HSD which inactivates teststerone was expressed in ORS, and this expression was stimulated with high calcium concentration. The mRNA of type III 17b-HSD which converts androstenedione to testosterone was strongly expressed in beard dermal papilla cells. Female axillary DPC showed stronger expression of type III 17b-HSD than that of male axillary DPC. Thease results suggesst that the sensitivity of hair androgen is patially controlled by the cell-type specific isozymes expression of 17b-HSD.
-196- Hair Biology(2) 9/5 Room-D
FIBROBLAST GROWTH FACTOR 10 EXPRESSION IN HUMAN HAIR FOLLICLE.
Kenji Hamada1,2, Kentaro Ozawa1, Satoshi Itami1, Kunihiko Yoshikawa1.
1Department of Dermatology, Osaka University Medical School, 2Research and Development Division, Rohto Pharmaceutical Co., Ltd., Osaka, Japan.
FGF-10, recently identified as a new member of the fibroblast growth factor family, is highly homologous to KGF. In order to study the possible involvement of FGF-10 in human hair growth, we examine the expression of human FGF-10 in dermal papilla cells, outer root sheath cells and keratinocytes in vitro. FGF-10 mRNA was expressed in human beard and frontal scalp dermal papilla cells. It was not detected in outer root sheath cells and keratinocyte in a low calcium medium, but was slightly expressed under high calcium medium in the both cells. The expression of FGF-10 mRNA was not affected by androgen in the dermal papilla cells. In vivo study, FGF-10 mRNA was detected in connective tissue sheath by in situ hybridization. These results suggest that FGF-10 is involved in the growth of human hair as a paracrine growth factor.
-197- Hair Biology(2) 9/5 Room-D
ULTRASONIC IMAGINGS IN PATIENTS WITH ALOPECIA.
Tetsuro Sugai.
Department of Dermatology, Ikeda Kaisei Hospital, Ikeda, Japan.
Ultrasonic imagings with dermascan C (Cotex Technology, Denmark) equipped a short B-probe were analyzed in 14 patients with alopecia areata and 2 patients with androgenic alopecia. Out of the 16 patients, male patients were 6 and female 10, ranging from 10 years to 65 years of age. Average age was 29.1 years. Ultrasonic imagings at a hairloss patch revealed regrown hairs, even at sites with the apparently no visible hairs. Ultrasonic imagings on various stages of hairloss patches are shown in comparison with their clinical findings.
-198- Hair Biology(2) 9/5 Room-D
PSYCHOSOMATIC SYMPTOMS, ANXIETY AND DEPRESSION IN PATIENTS WITH ALOPECIA AREATA.
Makoto Hashiro, Mutsuko Okumura.
Department of Dermatology, Kansai Rohsai Hospital, Hyogo, Japan.
Recently, it has been reported that some types of alopecia areata are autoimmune diseases. Nevertheless, for the treatment of patients with alopecia areata, the evaluation of their psychological state is necessary. We report here the psychological state of patients with alopecia areata and compare it with that of healthy controls. We used three kinds of psychological tests: Cornell medical index(CMI); state-trait anxiety inventory(STAI); and self-rating depression scale(SDS) for 20 patients and 29 controls. Patients with alopecia areata showed statistically higher scores for CMI(p¡ã0.05), state anxiety(p¡ã0.05) and trait anxiety ( p¡ã0.01), and tended to have a higher SDS score than healthy controls. However, no significant differences in the results of the three kinds of tests were seen amongthree levels of severity. In conclusion, patients with alopecia areata were significantly more psychosomatic symptom-prone and anxious, and tended to be more depressive than healthy controls. The severity of alopecia, however, was not found to affect the psychological state.